Table 2.
Primers used for characterization of Tyrophagus putrescentiae populations and detection of bacterial community.
| Specificity | Target | Name | Primer 5′–3′ | Tm (°C) | Length (bp) | Reference |
|---|---|---|---|---|---|---|
| T. putrescentiae -genomic | ITS2 region | 28S-F | CGACTTTCGAACGCATATTGC | 55 | 488 | Yang et al., 2011 |
| 28S-R | GCTTAAATTCAGGGGGTAATCTCG | |||||
| CO1 region | CO1-F | GTTTTGGGATATCTCTCATAC | 50 | 377 | Yang et al., 2011 | |
| CO1-R | GAGCAACAACATAATAAGTATC | |||||
| Bacteria | 16S rRNA | F27 | AGAGTTTGATCCTGGCTCAG | 50 | 1460 | Lane, 1991 |
| R1492 | TACGGYTACCTTGTTACGACTT | |||||
| Wolbachia | 16S rRNA | WpF | TTGTAGCCTGCTATGGTATAACT | 52 | 900 | O’Neill et al., 1992 |
| WpR | GAATAGGTATGATTTTCATGT | |||||
| Bartonella-like | 16S rRNA | Bart 1F | TGTCWCCGAYCCAGCCK | 63 | 920 | Kopecky et al., 2014a |
| Bart 2R | TGTCTCCGACCCAGCCT | |||||
| Cardinium | 16S rRNA | Card4 | CTTAACGCTAGAACTGCGA | 55 | 800 | Kopecky et al., 2013 |
| Card6 | TCAAGCTCTACCAACTCC | |||||
| 16S rRNA | Card1F | CGCATGCAATCTACTTTACAC | 55 | 1314 | This study | |
| Card1R | GCCACTGTCTTCAAGCTCTAC | |||||
| Blattabacterium | 16S rRNA | 35F | TGCAAGTCGAGGGGC | 62 | 1260 | Clark and Kambhampati, 2003 |
| 1294R | GTCGAGTTGCAGACTCCAATC | |||||
| Solitalea-like | 16S rRNA | Soli F | TGCGACACAAAGAGCTGA | 54 | 670 | This study |
| Soli R | GCTGGCAACAGTACATGG | |||||
| Spiroplasma | 16S rRNA | BS1 | AAGTCGAACGGGGTGCTT | 57 | 975 | Meeus et al., 2012 |
| BS976 | TGCACCACCTGTCTCAATGT |