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. 2016 Jul 12;7:999. doi: 10.3389/fpls.2016.00999

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Copyright © 2016 Lütz-Meindl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron microscopy (TEM) micrograph (A) of cell periphery and primary cell wall of M. denticulata with highlighted measurement sites of EEL spectra (scale bar is 1 μm). (B) EEL spectra acquired at the Ca L_2,3-edge, indicating calcium binding capacities of different cell components after doping with calcium acetate. Outer side of primary wall (green) has higher calcium binding capacity than inner side (red). Mucilage vesicle (blue) reveal highest calcium binding capacity, the cytoplasm (orange) lowest. Reprinted with permission from Eder and Lütz-Meindl (2008), Copyright^© 2008 Royal Microscopical Society.