Figure 1.

(A) Translocation model system. Two plasmids, one harboring a promoter, splicing donor, and PATRR11, and the other carrying PATRR22, a splicing acceptor, and a coding region of the GFP gene, were simultaneously transfected into HEK293 cells. After 24 h, fusion molecules generated by joining of the PATRR11 and PATRR22 at the center were detected by PCR or GFP-positive cells were monitored by flow cytometry (Inagaki et al., 2013). (B) Determination of the PATRR8 sequence by next-generation sequencing. Although the depth of the coverage was low at the center of the palindrome, massive parallel sequencing was able to fill the entire region of the palindrome (Mishra et al., 2014).