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. 2016 Jul 12;7:1088. doi: 10.3389/fmicb.2016.01088

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Copyright © 2016 Crozier, Hedley, Morris, Wagstaff, Andrews, Toth, Jackson and Holden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression overview. Heatmap of Escherichia coli O157:H7 (Sakai) total gene expression changes in response to different temperature and plant extract treatments. Changes in gene expression for E. coli O157:H7 (Sakai) grown for 1 h at 18°C are compared to cultures grown similarly at 37°C (37_MM), or at 18°C containing spinach (S. olercera) extracts of leaf lysates (Spin_LL) or root exudates (Spin_RE; A). Changes in gene expression for exposure to 1 h exposure to medium at 18°C containing polysaccharide extracts from spinach (S. olercera; Spin_PS) or lettuce (Lactuca sativa; Lett_PS) are compared to a baseline for E. coli O157:H7 (Sakai) in medium containing a no-plant control extract (‘Media’; B). Significant changes in expression of at least twofold are shown for induced (red) or repressed (green) genes.