Table 1.
Expression of selected genes encoding for hypothetical proteins of E. coli O157:H7 (Sakai).
| Accession # | MG1655 | EDL933 | Top BLASTn hit (nt identity %) | Gene expression data (fold-change) |
|||
|---|---|---|---|---|---|---|---|
| Spinach LL | Spinach RE | Spinach CWPS | Lettuce CWPS | ||||
| ECs0317 | ✓ | ✓ | Membrane protein, Escherichia albertii KF1 (97) | 11.85 | 4.81 | NS | NS |
| ECs0845 | x | ✓ | Bacteriophage tail protein HUN/2013, TL-2011C, Min27 (98) | NS | 5.00 | NS | NS |
| ECs0988 | ✓ | ✓ | yeaO, Shigella flexneri (99) | 2.49 | -18.16 | NS | 12.06 |
| ECs1254 | ✓ | ✓ | Putative enzyme, Shigella dysenteriae (90) | NS | NS | -4.92 | -8.98 |
| ECs1335 | x | x | Shigella flexneri plasmid pSFxv_1 (90) | 3.42 | NS | NS | 4.53 |
| ECs1375 | x | x | No highly similar BLAST hits outside Escherichia coli (n/a) | 2.87 | NS | NS | NS |
| ECs1653 | x | ✓ | Citrobacter freundii CFNIH1 (95) | -15.29 | 9.20 | NS | -13.23 |
| ECs1654 | x | ✓ | Citrobacter freundii CFNIH1 (94) | -32.08 | 8.79 | NS | -71.95 |
| ECs1655 | ✓ | ✓ | Citrobacter freundii CFNIH1 (94) | -18.13 | 8.85 | NS | -8.21 |
| ECs2304 | ✓ | ✓ | Shigella dysenteriae Sd197 (99) | 12.97 | 20.21 | NS | -3.27 |
| ECs2473 | x | ✓ | Escherichia albertii KF1 (81) | NS | NS | NS | 11.65 |
| ECs2489 | ✓ | ✓ | yeaD, Shigella dysenteriae Sd197 (99) | -3.60 | -2.96 | 2.50 | 3.30 |
| ECs2713 | x | x | Putative cytochrome, Shigella boydii (97) | -2.89 | NS | NS | -4.18 |
| ECs2940 | x | x | Bacteriophage tail fiber protein, Escherichia albertii KF1 (99) | NS | 4.72 | NS | NS |
| ECs3238 | x | ✓ | No highly similar BLAST hits outside Escherichia coli (n/a) | NS | 11.80 | NS | -2.29 |
| ECs3521 | ✓ | ✓ | FAD dependent oxidoreductase csiD, Shigella boydii (97) | -12.88 | NS | NS | -8.91 |
| ECs3750 | ✓ | ✓ | Conserved hypothetical protein, Shigella boydii Sb227 (99) | NS | 34.86 | NS | -12.47 |
| ECs4115 | ✓ | ✓ | aaeX, Shigella flexneri 2002017 (99) | 24.15 | 2.86 | NS | NS |
| ECs4474 | ✓ | ✓ | yibI, Shigella flexneri Shi06HN006 (99) | -3.10 | 267.91 | NS | -92.50 |
| ECs4491 | ✓ | ✓ | M23 peptidase domain protein, Shigella boydii CDC 308394 (98) | NS | -19.12 | NS | 10.08 |
| ECs4970 | x | x | Galactidol-1-phosphatol dehydrogenase, Citrobacter rodentium (94) | NS | NS | NS | 4.87 |
| ECs4976 | x | x | Galactidol-1-phosphatol dehydrogenase, Citrobacter rodentium (92) | NS | NS | NS | 7.13 |
| ECs5165 | ✓ | ✓ | Biofilm stress and motility protein A, Shigella flexneri Shi06HN006 (99) | -6.01 | 60.15 | NS | -7.03 |
Homologs in E. coli K-12 strain MG1655 and O157:H7 isolate EDL933 are indicated by ‘✓’ or ‘x’ for presence/absence and the top BLASTn hit provided with the percentage of nucleotide identity. The gene expression data from microarray analysis is provided as fold change (relative to the appropriate control; NS is not significant), for each of the four plant extracts: spinach leaf lysates (LL) and root exudates (RE); and cell wall polysaccharides (CWPSs) from spinach and lettuce.