FIGURE 4.
Effect of serelaxin on (A) mRNA and (B) protein expression of Col1A1 as well as (C) protein expression of total collagen in kidney tissue of WT and cGKI-KO mice. mRNA was determined with RT-qPCR and 18s rRNA as housekeeping gene. Results are shown as fold change of mRNA expression (2ΔΔct) in the fibrotic kidney relative to contralateral healthy kidney, which was set as 1. The increase of Col1A1 protein expression [%] in fibrotic renal tissue compared to contralateral kidney was quantified by immunohistochemistry; the increase of total collagen [%] was quantified with sirius red/fast green method; cGKI, cGMP-dependent protein kinase I; Col1A1, collagen1A1; KO, knock out; RLX, serelaxin; UUO, unilateral ureteral obstruction; WT, wildtype. ∗p < 0.05, ∗∗p < 0.01.