Table 3. Cycle threshold (Ct) values for triplicate sputum and bronchoalveolar lavage (BAL) samples following processing using three different homogenization methods.
| Homogenization by | N* | Sputum | BAL | ||
|---|---|---|---|---|---|
| upE | ORF1a | upE | ORF1a | ||
| PK-DNase 1 | 3 | 34.9/36.3/34.0 | 35.0/34.9/35.4 | 34.1/33.9/34.4 | 34.6/34.0/35.1 |
| PBS | 3 | Neg/37.9/Neg | Neg/Neg/Neg | 34.1/33.9/33.7 | 34.8/34.4/35.0 |
| NALC-sodium citrate | 3 | Neg/Neg/Neg | Neg/Neg/Neg | 35.0/34.6/34.3 | 35.7/35.8/35.5 |
*Three different sputum and bronchoalveolar lavage samples were included, and results are provided in this order.
Abbreviations: NALC, N-acetyl-L-cysteine; Neg, negative; PBS, phosphate-buffered saline; PK, proteinase K.