Table 1.
Chronic mild stress schedule.
| Time (hours) | First Wed. | Thr. | Fri. | Sat. | Sun. | Mon. | Tue. | Every subsequent Wed. |
|---|---|---|---|---|---|---|---|---|
| 7:00-8:00 | SC/CL | WD | O/CL | FD/WD | ||||
| 8:00-9:00 | SC/CL | WD | CT/CL | FD/WD | ||||
| 9:00-10:00 | CL | WD | CT/CL | FD/WD | ||||
| 10:00-11:00 | Baseline | SL | WN | CT/CL | SPT | |||
| 11:00-12:00 | O | SL | WN | CT/CL | ||||
| 12:00-13:00 | O | SL | WN | SL | CT | |||
| 13:00-14:00 | WN | O | SL | WN | SL | CT | WN | |
| 14:00-15:00 | WN | O | SL | SL | FD/WD | WN | ||
| 15:00-16:00 | WN | O/CL | SL | FD/WD | WN | |||
| 16:00-17:00 | SC/CL | WD | SL | FD/WD | ||||
| 17:00–7:00 | SC/CL | WD | O/CL | FD/WD |
WN: white noise (~90 dB); O: overcrowding (2-3 rats per cage); CL: continuous lighting; SC: soiled cage (250 mL water spilled into bedding); SL: stroboscopic light (300 flashes/min); WD: water deprivation; CT: cage tilt (45°); FD: food deprivation; SPT: sucrose preference test.