Figure 7.

HSYA inhibits NLRP3 inflammasome activation. RAW264.7 cells were pretreated with HSYA at indicated doses (25, 50, and 100 μM) for 3 h and then treated with 1 μg/mL LPS for 8 h. The GAPDH, NLRP3, ASC, procaspase-1, and caspase-1 proteins expressions were analyzed by Western Blotting. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. ^∗ P < 0.05, ^∗∗ P < 0.01, ^$$ P < 0.01, ^## P < 0.01, ^△ P < 0.05, and ^△△ P < 0.01 indicate significant differences compared with the control group of indicated proteins, respectively.