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. 2016 Jun 28;2016:8172706. doi: 10.1155/2016/8172706

Figure 8.

Figure 8

HSYA inhibits LPS-induced IL-1β and IL-18 secretion. RAW264.7 cells were pretreated with HSYA at indicated doses (25, 50, and 100 μM) for 3 h and then treated with 1 μg/mL LPS for 12 h (Western Blot) or 24 h (ELISA). (a) The pro-IL-1β, IL-1β, and GAPDH proteins expressions were analyzed by Western Blotting. (b) The IL-1β and IL-18 secretion were measured by ELISA. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. ∗ P < 0.05, ∗∗ P < 0.01, # P < 0.05, and ## P < 0.01 indicate significant differences compared with the control group of indicated proteins, respectively.