Table 1.

Parameter Values for the Reference Parameter Set

Symbol	Parameter	Reference Value	Notes
v	motor speed	0.5 μm s−1	measured by Bieling et al. (8); varied up to 8 μm s−1 to study effect of varying speed on density profiles
$k_{\text{on}}$	binding rate constant	2.7 × 10−4 nM−1 s−1	estimated based on motor density profiles and kymographs to give affinity of 6.3 nM, ∼15× higher than measured for motor on single MT by Bieling et al. (42)
c	bulk motor concentration	1–200 nM	varied by Bieling et al. (8)
$k_{\text{off}}$	unbinding rate	0.169 s−1	measured by Bieling et al. (8)
s	switching rate	0.44 s−1	measured by Bieling et al. (8)
α	motor flux constant into overlap from MT minus end	0	motors bind primarily inside the overlap; see discussion in the Results; we varied α between 0 and 1 to determine the model phase diagram
β	motor flux constant out of overlap from MT plus end	0	an upper bound on the end motor unbinding rate is β = 2.7 × 10−3; see discussion in the Results; we varied β between 0 and 1 to determine the model phase diagram
N	number of sites	120–2500	varied to study overlaps of length 1–20 μm
δ	length of a single site	8 nm	length of an α-β tubulin dimer; see Bray (1)