Figure 4.

Behavioural effects of β‐arrestin 2 super‐recruiters like SNC80 are β‐arrestin 2 dependent. (A) The ability of SNC80 (20 mg⋅kg⁻¹, s.c.) to increase alcohol intake is abolished in transgenic β‐arrestin 2 KO mice. In contrast, TAN‐67 (25 mg⋅kg⁻¹, s.c.) still decreased alcohol intake in β‐arrestin 2 KO mice. (B) SNC80 (20 mg⋅kg⁻¹, s.c.) did not alter the expression of alcohol place preference in β‐arrestin 2 KO mice. (C) SNC80 (20 mg⋅kg⁻¹, s.c.) did not affect alcohol metabolism in wild‐type and β‐arrestin 2 KO mice. (D) β‐Arrestin 2 KO did not change SNC80 (20 mg⋅kg⁻¹, s.c.) induced hyperlocomotion, or induce locomotor activity to TAN‐67 (25 mg⋅kg⁻¹, s.c.). (E) The ability of SNC80 (20 mg⋅kg⁻¹ s.c.) and to a lesser extent TAN‐67 (25 mg⋅kg⁻¹ s.c.) to reduce depression‐like behaviour was attenuated in β‐arrestin 2 KO mice. The group size for each drug is depicted inside the column of that drug. Significance was determined by two‐way anova followed by a Bonferonni post hoc test. *P < 0.05, ***P < 0.001, ****P < 0.0001.