Fig. 5.

Binding of STAT3 to the BDNF, PICK1, and synaptophysin promoters

The ChIP of synchronized neocortical cells was performed using an antibody to STAT3, a negative control antibody (IgG), or a positive control anti-RNA polymerase (anti-RP). A sample without DNA was tested as an additional control for DNA contamination, and an input sample was also detected. Immunoprecipitated DNA analyzed by PCR using primers that amplify the BDNF (a), PICK1 (b), synaptophysin (c) promoters and GAPDH gene (d), respectively