Figure 2.

Phylogenetic tree of terpene synthases (TPS) with confirmed capacity for multi-substrate use (Table 1 for details of product and substrate specificities). The red branch denotes TPS with C5/C10 activity, the black branches with C10/C15 activity, the blue branches with C10/C15/C20 activity, and the green branches with C15/C20 activity. These 40 multi-substrate terpene synthase are from different TPS families including TPS-a, TPS-b, TPS-g, TPS-d, TPS-e, and TPS-f. The tree was constructed by MEGA5 software by UPGMA method (Tamura et al., 2011). The asterisks denote the presence of the conserved arginine-rich RRx8W motif at the N-terminal of the protein that is common in many monoterpene synthases (Chen et al., 2011). The underlined enzymes demonstrate the presence of transit peptide.