Table 1.
Primer sequences for PCR analysis
| Repeat sequence | mtDNA region | Position | Annealing(°C) | Primer(5’-3’) |
|---|---|---|---|---|
| (C)n | 270-425 | D-loop | 58 | TCCACACAGACATCAATAACA |
| AAAGTGCATACCGCCAAAAG | ||||
| (CA)n | 467-556 | D-loop | 55 | CCCATACTACTAATCTCATCAA |
| TTTGGTTGGTTCGGGGTATG | ||||
| (C)6 | 3529-3617 | ND1 | 55 | CCGACCTTAGCTCTCACCAT |
| AATAGGAGGCCTAGGTTGAG | ||||
| (A)7 | 4555-4644 | ND2 | 55 | CCTGAGTAGGCCTAGAAATAAA |
| ACTTGATGGCAGCTTCTGTG | ||||
| (T)7 | 9431-9526 | C0III | 55 | CCAAAAAGGCCTTCGATACG |
| GCTAGGCTGGAGTGGTAAAA | ||||
| (C)6 and(A)8 | 12360-12465 | ND5 | 55 | CACCCTAACCCTGACTTCC |
| GGTGGATGCGACAATGGATT | ||||
| (CCT)3 and(AGC)3 | 12940-13032 | ND5 | 55 | GCCCTTCTAAACGCTAATCC |
| TCAGGGGTGGAGACCTAATT |