. 2016 Jul 11;4:72. doi: 10.1186/s40478-016-0341-4
© The Author(s). 2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
| Step # | Temperature (°C) | Time (minutes) | Comments |
| 1 | 50 | 2 | - |
| 2 | 94.5 | 10 | - |
| 3 | 97 | 0.5 | X40 repeats |
| 4 | 59.7 | 1 |