| Step # | Temperature (°C) | Time (minutes) | Comments |
| 1 | 95 | 20 | - |
| 2 | 95 | 0.5 | X40 repeats |
| 3 | 60 | 1.5 | |
| 4 | 95 | 15 | - |
| 5 | 60 | 15 | - |
| 6 | 95 | 15 | - |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.