| Injection |
injected eggs |
2569 |
|
|
|
|
|
|
|
|
|
|
|
| |
hatched larvae |
694 |
|
|
|
|
|
|
|
|
|
|
27 |
| G0 |
G0 T7EI genotyped larvae |
58 |
|
|
|
|
|
|
|
|
|
|
|
| |
G0 mutated larvae (T7EI gel) |
52 |
|
|
|
|
|
|
|
|
|
|
89.6 |
| |
G0 sequenced larvae |
33 |
|
|
|
|
|
|
|
|
|
|
|
| |
G0 larvae with multiple mutations (uncharacterized sequences) |
17 |
|
|
|
|
|
|
|
|
|
|
|
| |
G0 larvae with multiple mutations (characterized sequences) |
7 |
6,7,9,10,11,13,14 |
|
|
|
|
|
|
|
|
|
|
| |
G0 larvae with unique mutation (characterized sequence) |
9 |
1–5,8,12,15,16 |
|
|
|
|
|
|
|
|
|
|
| |
G0 sequence mutations provoking ORF frameshifts |
11 |
2–7,9,11,14,15,16 |
|
|
|
|
|
|
|
|
|
|
| Founders |
G0 mutant lines tested for founder effects (mutation transmission) |
10 |
1–6,9,11,15,16 |
|
|
|
|
|
|
|
|
|
|
| |
G0 founders |
8 |
1,3–6,9,15,16 |
|
|
|
|
|
|
|
|
|
80 |
| G1 genotyping |
Mutated sequences (as in
Fig. 3) |
1 |
2 |
3 |
4 |
5 |
6 |
9 |
11 |
15 |
16 |
|
|
| |
G1 genotyped larvae (T7EI) |
30 |
30 |
30 |
30 |
30 |
30 |
30 |
30 |
30 |
30 |
300 |
|
| |
G1 heterozygote larvae (T7EI gel) |
10 |
10 |
5 |
6 |
10 |
5 |
11 |
11 |
9 |
13 |
33 |
|
| |
G1 heterozygote larvae (sequencing) |
10 |
0 |
3 |
2 |
7 |
5 |
11 |
0 |
6 |
13 |
57 |
|
| Germline mutation rate |
% heterozygote larvae (sequencing) |
33.3 |
0 |
10 |
6.6 |
23.3 |
16.6 |
36.6 |
0 |
20 |
43.3 |
19 |
|
| G2 |
G2 genotyped larvae (T7E1) |
61 |
|
|
|
60 |
|
|
|
59 |
64 |
244 |
100 |
| |
G2 +/+ larvae (T7EI) |
18 |
|
|
|
23 |
|
|
|
19 |
18 |
78 |
32 |
| |
G2 −/+ larvae (T7EI) |
40 |
|
|
|
37 |
|
|
|
37 |
40 |
154 |
63 |
| |
G2 −/− larvae (T7EI, verified by sequencing) (see Table 2 for details) |
3 |
|
|
|
0 |
|
|
|
3 |
6 |
12 |
5 |
