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Gel mobility shift assay. Nuclear extracts of Chicken skeletal muscle cells pre‐incubated with anti‐Nished antibody (Nished) or pre‐immune serum (PI) were incubated with radiolabelled CSS oligonucleotide and subjected to gel mobility shift assay as described in ‘Materials and Methods’. □ denotes the DNA‐protein complexes disrupted by anti‐Nished antibody.
