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. 2008 Oct 13;13(8b):1952–1961. doi: 10.1111/j.1582-4934.2008.00525.x

Figure 5.

Figure 5

Western blot of total cell lysates from C2C12 cells stably transfected with pcDNA6 vector (V6) or with plasmid expressing Nished (N1). (A) cell lysates were immunoprecipitated and blotted with anti‐Nished antibody or pre‐immune serum. As loading control we used equal amount of proteins from each cell line, and by Western blot determined the GAPDH levels. (B) Transient transfection of plasmid pMutIRELuc in C2C12 cells stably transfected with pNished (N1) or vector alone (V5). Data are expressed as percentage activity of pMutIRELuc transfected in wild‐type C2C12 cells. (n= 4 in triplicate; □, one sample t‐test; P < 0.05).