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. 2008 Oct 13;13(8b):1952–1961. doi: 10.1111/j.1582-4934.2008.00525.x

Figure 7.

Figure 7

Tricostatin A releases CSS mediates repression of MLC2v promoter in C2C12 cells. (A) C2C12 cells were transfected with MLC2v promoter containing mutated IRE (pMutIRELuc). After 24 hrs, cells were treated with Tricostation A (TSA), an inhibitor of Class I and II HDACs, and 25 μM each of Sirtinol and M15, inhibitors of Class III HDACs. The HDACs inhibitors were dissolved in DMSO. Luciferase assays were performed 18 hrs after treatment. Nished repression of MLC2v promoter was relieved by TSA in a dose‐dependent manner, whereas Sirtionol and M15 had no effect. (B) Similar transient transfection experiment was performed in chicken primary cardiac cells, however, TSA treatment failed to modify the MLC2v promoter activity (n= 4 in triplicate, □ one sample t‐test, P < 0.05).