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. 2009 Jul 6;13(8b):2293–2303. doi: 10.1111/j.1582-4934.2009.00838.x

Figure 1.

Figure 1

Acute exposure to high concentrations of DCA stimulates Cl− secretion across T84 cells. (A) Voltage‐clamped monolayers of T84 cells were bilaterally exposed to DCA (1 mM) and Isc responses were recorded (n= 5). (B) Voltage‐clamped T84 cells were exposed to increasing concentrations of bilateral DCA and maximal Isc responses were measured (n= 5). (C) Monolayers of T84 cells were exposed to increasing concentrations of DCA for 20 min after which TER was measured (n= 3). (D) Aliquots of supernatants from cells exposed to DCA at varying concentrations for 20 min were analysed for LDH release. Data are expressed as sample absorbance at 490 nm (n= 3). (E) TER was measured across T84 cell monolayers exposed to 500 μM DCA (bilateral). In one set of monolayers DCA was removed after 40 min (n= 4). *P < 0.05; **P < 0.01; ***P < 0.001.