Figure 5.

The antisecretory effect of DCA is not mediated by muscarinic receptors or by alterations in agonist‐induced generation of the second messengers. (A) Cells were pretreated with atropine (1 μM) for 30 min prior to DCA (100 μM). After a further 24 hrs incubation, cells were washed and I_sc responses to FSK (10 μM) were measured (n= 5). (B) T₈₄ cells grown on glass coverslips were pretreated for 24 hrs with DCA (50 μM) after which CCh‐induced mobilization of intracellular Ca²⁺ was measured by Fura‐2 fluorescence. Data are expressed as mean fluorescence ratio at 340 and 380 nM (n= 3 coverslips for each condition). The inset shows the net change in mean fluorescence ratio (ΔF_340/380) for these experiments. (C) Monolayers of T₈₄ cells were treated with DCA (50 μM) for 24 hrs and then stimulated with FSK (10 μM) for 5 min, lysed, and intracellular cAMP was measured (n= 4). **P < 0.01.