Table 1.
Comparison of TCID50 titers of influenza A and B virus on ELVIRA Flu A‐luc and Flu B‐Rluc cells and MDCK cells
| Virus strain | Method* | |
|---|---|---|
| ELVIRA Flu A Luc | MDCK | |
| A | ||
| A/Brisbane/59/2007 (H1N1) | 1·08E + 07 | 1·26E + 07 |
| A/Solomon island/03/2006 (H1N1) | 1·58E + 07 | 1·99E + 07 |
| A/Wisconsin/56/2005 (H3N2) | 1·80E + 07 | 1·19E + 07 |
| A/Mal/302/54 (H1N1) | 2·59E + 07 | 8·89E + 06 |
| A/Whooperswan/Mongolia/ 244/05 (H5N1) | 1·78E + 08 | 1·2E + 08 |
| A/Hong Kong/483/97 (H5N1) | 5·01E + 07 | 2·51E + 06 |
| A/Hong Kong/486/97 (H5N1) | 2·09E + 08 | 1·17E + 07 |
| A/Vietnam/1203/04 (H5N1) | 2·57E + 07 | 3·31E + 07 |
| B | ||
| B/Malaysia2506/04 | 5·00E + 07 | 2·32 E + 07 |
| B/GL/1739/54 | 5·00E + 05 | 1·25 E + 06 |
| B/Florida04/2006 | 5·00E + 06 | 2·59 E + 06 |
*Ten‐fold serial dilutions of influenza A or B virus stocks were made in serum free medium. ELVIRA Flu A and MDCK cells were planted in 96‐well plates and then infected with the 10−4 through 10−9 dilutions (10 wells/dilution). After infection, the cells were incubated at 37°C with 5% CO2 for 24 or 48 hours (ELVIRA cells) or 4 days (MDCK cells). ELVIRA Flu A luc and ELVIRA Flu B Rluc wells with a RLU reading of >3 times the background mean were considered positive for influenza infection. MDCK cells were inspected microscopically for cytopathic effects. TCID50 was calculated by the Reed & Muench method.