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. 2016 Jul 11;11:63. doi: 10.1186/s13000-016-0511-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Probability of PCa RFS and PCSM for ERβ2 staining quantified by image analysis. Kaplan-Meier plot for PCa recurrence-free survival using tertiles of ERβ2 intensity quantified by the Cytoplasm algorithm (Aperio) confined to tumor cytoplasm (a), tumor nuclei (c), or by the Color Deconvolution algorithm (Aperio) for area-based quantification confined to tumor cells including cytoplasm and nuclear staining (e). Kaplan-Meier plot for PCa-specific survival using tertiles of ERβ2 intensity quantified by the Cytoplasm algorithm (Aperio) confined to tumor cytoplasm (b), tumor nuclei (d), or by the Color Deconvolution algorithm (Aperio) for area-based quantification confined to tumor cells including cytoplasm and nuclear staining (f)