Figure 2.

(a) Detection of AAV9_3XFLAG-TALE_frat#8-VP64 transgene and protein in the muscle, heart and liver of YG8R mice. Muscle (M), liver (L) and heart H) were removed from 4-month-old mice treated with 6 × 10¹¹ vg (left panel), from 9-day-old mice treated with 6 × 10¹² vg (right panel) or from control mice injected with saline (middle panel). The expected 446 pb PCR product was strongly detectable in the liver but barely detectable in the muscles and heart of 4-month-old mice. The amplicon was also strongly detected in the three tissues of the 9-day-old mice. (b) Proteins extracted from the liver of 4-month-old (left panel) and from the heart of 9-day-old (right panel) YG8R mice were loaded on a 10% SDS-polyacrylamide gel. In both panels, the left lane contains proteins from a control mouse treated with saline and the right lane contains proteins from mice injected intraperitoneally with AAV9_3XFLAG-TALE_frat#8-VP64. The transgenic protein (90 kDa) was detected by western blot with a monoclonal antibody against the 3XFLAG in tissues of treated mice but not in control tissues.