Fig. 5.

Detection of PIK3CA, KRAS and BRAF mutations by individual PCR-RFLP methods in CRC samples. a: Detection of the PIK3CA mutation using our PCR-RFLP. b: Detection of the KRAS mutation using PCR-RFLP. A 107-bp fragment of KRAS was amplified, followed by digestion with MvaI and analysis by 3 % agarose gel electrophoresis. The MvaI digestion of wild-type KRAS yielded two 77- and 30-bp bands, while the mutant-type remained intact (107 bp). c: Detection of the BRAF mutation using PCR-RFLP. A 224-bp fragment of BRAF was amplified, and followed by digestion with TspRI and analysis by 3 % agarose gel electrophoresis. The TspRI digestion of wild-type BRAF yielded three 124-, 87- and 13-bp bands, while the mutant-type yielded two 211- and 13-bp bands. M: DL500 DNA marker