Fig. 5. EZH2/H3K27me3 influence cell cycle in the SVZ cells.

(A) Scheme of coronal sectioned mouse brain indicates region of immunostaining. Multiple images of dorsal SVZ and RMS were taken along rostro-caudal axis at 20X and 40X to obtain representative images of DAPI – positive and EdU-positive nuclei.

(B) Bargraph of EdU-positive nuclei within dorsal SVZ quantified using 40× magnification. Y-axis indicates percentage of cells which are EdU-positive compared to control.

(C) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse with wildtype Ezh2 allele 10 days post-stereotaxic Cre administration. 20X, Scale bar = 20 um.

(D) 10 days post-stereotaxic Cre administration into a R26RLacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. Cells undergoing DNA synthesis during the 2 hour window are detected as EdU -positive nuclei at dorsal SVZ and rostral migratory stream. 20X, Scale bar = 20 um.

(E) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene. 20X, Scale bar = 20 um.

(F) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. The proportion of EdU-positive nuclei at the dorsal horn of the SVZ and RMS are diminished following stereotaxic injection into dorsal SVZ compared to Cre-injected R26RLacZ reporter mice. 20X, Scale bar = 20 um.

(G) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse with wildtype Ezh2 allele 10 days post-stereotaxic Cre administration. 40X, Scale bar = 20 um.

(H) 10 days post-stereotaxic Cre administration into a R26RLacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. Cells undergoing DNA synthesis during the 2 hour window are detected as EdU -positive nuclei at dorsal SVZ and rostral migratory stream. 40X, Scale bar = 20 um.

(I) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene. 40X, Scale bar = 20 um.

(J) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. The proportion of EdU-positive nuclei at the dorsal horn of the SVZ and RMS are diminished following stereotaxic injection into dorsal SVZ compared to Cre-injected R26RLacZ reporter mice. 40X, Scale bar = 20 um.

(K) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse 10 days post-stereotaxic Cre administration. 20X, Scale bar = 20 um.

(L) 10 days post-stereotaxic Cre administration into a R26RLacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. 20X, Scale bar = 20 um.

(M) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse. 20X, Scale bar = 20 um.

(N) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. 20X, Scale bar = 20 um.

(O) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse 10 days post-stereotaxic Cre administration. 40X, Scale bar = 20 um.

(P) 10 days post-stereotaxic Cre administration into a ROSA-LacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.

(Q) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse. 40X, Scale bar = 20 um.

(R) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.

(S) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse 10 days post-stereotaxic Cre administration. 20X, Scale bar = 20 um.

(T) 10 days post-stereotaxic Cre administration into a R26RLacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. 20X, Scale bar = 20 um.

(U) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse. 20X, Scale bar = 20 um.

(V) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. 20X, Scale bar = 20 um.

(W) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream in R26RLacZ reporter mouse 10 days post-stereotaxic Cre administration. 40X, Scale bar = 20 um.

(X) 10 days post-stereotaxic Cre administration into an R26RLacZ reporter mouse with wildtype Ezh2 allele, EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.

(Y) DAPI counterstain depicts nuclei at dorsal SVZ and rostral migratory stream 10 days post-stereotaxic Cre administration in an Ezh2^flox/flox:ROSA^L/L knockout mouse. 40X, Scale bar = 20 um.

(Z) 10 days post-stereotaxic Cre administration into an Ezh2^flox/flox:ROSA^L/L knockout mouse with 2 copies of a floxed Ezh2 gene, EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.