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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Neuroepigenetics. 2016 May 3;6:10–25. doi: 10.1016/j.nepig.2016.04.001

Fig. 6. Suv4-20h/H4K20me3 influence cell cycle in the SVZ cells.

Fig. 6

(A) Scheme of coronal sectioned mouse brain indicates region of immunostaining.

(B) Co-localization of EdU and DAPI in non-injected SVZ in Suv4-20hflox/flox:RosaY/Y mouse. 20X, Scale bar = 20 um.

(C) Co-localization of EdU and DAPI in Cre injected SVZ in Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic administration. 20X, Scale bar = 20 um.

(D) Bargraph of EdU-positive nuclei within dorsal SVZ quantified using 40x magnification. Y-axis indicates percentage of cells which are EdU –positive compared to control.

(E) Non-injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse depicting DAPI at dorsal SVZ and rostral migratory stream. 20X, Scale bar = 20 um.

(F) Non-injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse. EdU was detected 2 hours after intraperitoneal EdU injection. 20X, Scale bar = 20 um.

(G) Cre injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration depicting DAPI at dorsal SVZ and rostral migratory stream. 20X, Scale bar = 20 um.

(H) Cre injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration into. EdU was detected 2 hours after intraperitoneal EdU injection on last day of post-surgical rest. 20X, Scale bar = 20 um.

(I) Non-injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse depicting DAPI at dorsal SVZ and rostral migratory stream. 40X, Scale bar = 20 um.

(J) Non-injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse. EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.

(K) Cre injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration depicting DAPI at dorsal SVZ and rostral migratory stream. 40X, Scale bar = 20 um.

(L) Cre injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration into. EdU was detected 2 hours after intraperitoneal EdU injection on last day of post-surgical rest. 40X, Scale bar = 20 um.

(M) Non-injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse depicting DAPI at dorsal SVZ and rostral migratory stream. 20X, Scale bar = 20 um.

(N) Non-injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse. EdU was detected 2 hours after intraperitoneal EdU injection on last day of post-surgical rest. 20X, Scale bar = 20 um.

(O) Cre injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration depicting DAPI at dorsal SVZ and rostral migratory stream. 20X, Scale bar = 20 um.

(P) Cre injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration into. EdU was detected 2 hours after intraperitoneal EdU injection on last day of post-surgical rest. 20X, Scale bar = 20 um.

(Q) Non-injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse depicting DAPI at dorsal SVZ and rostral migratory stream. 40X, Scale bar = 20 um.

(R) Non-injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse. EdU was detected 2 hours after intraperitoneal EdU injection. 40X, Scale bar = 20 um.

(S) Cre injected hemisphere in Suv4-20hflox/flox:RosaY/Y mouse, 5 days post-stereotaxic Cre administration depicting DAPI at dorsal SVZ and rostral migratory stream. 40X, Scale bar = 20 um.

(T) Cre injected hemisphere in a Suv4-20hflox/flox:RosaY/Y mouse. EdU was detected 2 hours after intraperitoneal EdU injection on last day of post-surgical rest. 40X, Scale bar = 20 um.