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. 2016 Feb 2;65(7):1202–1214. doi: 10.1136/gutjnl-2015-310798

Figure 5.

Figure 5

Hepatocyte and extrahepatocyte peroxisome proliferator-activated receptor α (PPARα) regulate fibroblast growth factor 21 (FGF21), glycaemia and body temperature during fasting. (A and B) Eleven-week-old male mice of the C57Bl/6J background were fed ad libitum or fasted for 24 h, and were killed around the clock from ZT0 to ZT24. (A) Fgf21 mRNA was quantified by qRT-PCR. (B) Quantification of circulating FGF21 levels by ELISA. (C) Twelve-week-old wild-type (WT), PPARα-hepatocyte knockout (Pparαhep−/−) and PPARα knockout (Pparα−/−) male mice were fed ad libitum or fasted for 16 h and blood was collected at ZT8 (ZT8 fed) or at ZT16 (ZT16 fasted). FGF21 plasma level was determined by ELISA. (D–G) Male mice of WT, Pparαhep−/− and Pparα−/− genotypes were infected with an adenoviral construct containing cDNA of Fgf21 or an empty vector. Mice were sacrificed after a 24 h fasting period at ZT14. (D) Quantification of circulating FGF21 levels by ELISA. (E) Fgf21, G6pd and Scd1 mRNAs were quantified by qRT-PCR. (F) Quantification of hepatic cholesterol esters and triglycerides. (G) Representative pictures of H&E staining of liver sections. Scale bars, 100 µm. (H) Plasma glucose level was monitored over a 24 h fasting period from ZT0 to ZT24 in WT, Pparαhep−/− and Pparα−/− mice. (I, J) Plasma glucose (I) and body temperature (J) were determined at ZT0 in fed mice or at ZT0 in mice fasted for 24 h. Data are shown as mean±SEM. *p≤0.05, **p≤0.01, ***p≤0.005.