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. 2016 Feb 2;65(7):1202–1214. doi: 10.1136/gutjnl-2015-310798

Figure 6.

Figure 6

Hepatocyte peroxisome proliferator-activated receptor α (PPARα) activity is induced by adipose tissue lipolysis. (A and B) Liver samples were collected from male wild-type (WT) C57Bl/6J mice that were fed ad libitum (black curve) or fasted (blue curve) over 24 h. (A) Hepatic mRNA expression levels of Pparα, Cyp4a14, Vnn1, Cyp4a10, Fsp27 and Tnfα were quantified by qRT-PCR. (B) Plasma glucose and free fatty acids (FFA) were measured. (C and D) WT and PPARα hepatocyte-specific knockout (Pparαhep−/−) mice were treated with the β3-adrenergic receptor agonist CL316243 at ZT6 and then killed at ZT14. (C) Quantification of plasma FFA. (D) Relative mRNA expression levels of Fgf21, Cyp4a14, Vnn1, Cyp4a10 and Fsp27 were measured by qRT-PCR. Data are shown as mean±SEM. *p≤0.05, **p≤0.01, ***p≤0.005.