Skip to main content
. 2015 Jun 1;65(7):1151–1164. doi: 10.1136/gutjnl-2014-308270

Figure 5.

Figure 5

B-cell CLL/lymphoma 3 (BCL-3) expression promotes cell survival in hypoxic growth conditions. (A) SW480 cells transfected with BCL-3 expression or control plasmid were placed in 1% O2 for 48 h. Hypoxia significantly decreased cell yield and increased apoptosis in control plasmid-transfected cells. BCL-3 overexpression in 1% O2 decreased cell death to that observed for control plasmid-transfected cells in normoxia. Western analysis showed successful overexpression of BCL-3 in 1% O2. Expression of HIF-1α was detected in cells placed in 1% O2. Total AKT levels increased in 1% O2 (only seen in this cell line). AKT phosphorylation at S473 was increased in cells overexpressing BCL-3 in 1% O2 as well as normoxia. (B) HCT116 cells were transfected with BCL-3 or negative small interfering (si)RNA were placed in 1% O2 for 48 h. AKT phosphorylation at S473 was decreased in BCL-3 siRNA-transfected cells in hypoxia as well as normoxia, while levels of total AKT remained unchanged. Western analysis showed successful suppression of BCL-3 in 1% O2.