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. 2016 Jul 12;4:16014. doi: 10.1038/boneres.2016.14

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Copyright © 2016 The Author(s)

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Binding assay of NIRF probe cFLFLF-PEG-Cy7 to RAW264.7 cells stimulated by 1 μg·mL⁻¹ lipopolysaccharide vs no treatment (NT).⁹⁰ (a) Various concentrations of the probe were applied to the assay, including (from top to bottom) 1.0, 0.5, 0.1 and 0.05 μmol·L⁻¹. Results indicate that fluorescent intensity strengthened with increasing doses of the probe, and that LPS was able to enhance binding affinity of the probe for cells. (b) A blocking test was performed with cFLFLF and a Scramble peptide (cLFFFL), revealing that the binding signal was blocked by cFLFLF but not controls.