Study Type	Result	Reference
Synthesis.	Kahalalide F was first isolated from the marine mollusk (Elysia rufescens) and then directly from its algal diet (Bryopsis pennata). In both cases ethanol extracts were fractioned by silica flash column chromatography and the eluted peptide mixtures were fractioned by repeated HPLC to yield kahalalide F.	506285
SAR.	SAR data of kahalalide A to G revealed that, of all the peptides, only kahalalide F had significant biological activity that was lost when its ester bond was hydrolyzed and this cyclic compound was transformed to its linear form kahalalide G.	526209
Synthesis.	Total synthesis of kahalalide F revealed that the stereochemistry of the linear side chain, particularly of valines 3 and 4, was essential for biological activity.	506272