Table 1.

Bacterial strains and plasmids.

Strains or plasmids	Relevant characteristicsa	Reference or sourceb
Strains
C. acetobutylicum ATCC 824	Wild type	ATCC
E.coli ER2275	hsdR mcr recA1 endA1	NEB
E.coli DH5α	General cloning host strain	Takara
Plasmids
pAN1	Φ3TI, p15a origin, Sper	Mermelstein and Papoutsakis (1993)
pMTL82254	Clostridium modular plasmid with catP reporter, pBP1 (Gram+ origin), ColE1+tra (Gram– origin), Emr	Heap et al. (2009)
pMTL82254-Pfdx	Clostridium modular plasmid with catP reporter expressed by the fdx promoter, pBP1 (Gram+ origin), ColE1+tra (Gram– origin), Emr	Zhang, Grosse-Honebrink and Minton (2015)
pMTL82254-Pcac1339	Clostridium modular plasmid with catP reporter expressed by promoter of cac1339, pBP1 (Gram+ origin), ColE1+tra (Gram– origin), Emr	This study
pMTL82254-Pcac1344	Clostridium modular plasmid with catP reporter expressed by promoter of cac1344, pBP1 (Gram+ origin), ColE1+tra (Gram– origin), Emr	This study
pMTL82254-Pcac2612	Clostridium modular plasmid with catP reporter expressed by promoter of cac2612, pBP1 (Gram+ origin), ColE1+tra (Gram– origin), Emr	This study
pMTL83151	Clostridium modular plasmid used for construction of IPTG inducible promoter system, pCB102 (Gram+ origin), ColE1+tra (Gram– origin), Tmr	Heap et al. (2009)
pMTL-SC0	Transposon plasmid with pBP1 replicon, Tmr	Cartman and Minton (2010)
pMTL-YG0	Derived from pMTL-SC0 by replacing pBP1 with pCB102 replicon	This study
pMTL-YG3	Derived from pMTL-YG0 by introducing the promoter of cac1339 to express the transposase Himar1 C9	This study

^a hsdR, host-specific restriction deficient; mcr, methylcytosine-specific restriction abolished; recA1, homologous recombination abolished; endA1, endonucleases abolished; Spe^r, spectinomycin resistance; Em^r, erythromycin resistance; Tm^r: thiamphenicol resistance; pBP1, Gram-positive origin of replication; pCB102, Gram-positive origin of replication, which was unstable in C. acetobutylicum

^bATCC, American Type Culture Collection; NEB, New England Biolabs.