Table 2.
Oligonucleotide primers used in this study.
| Primer name | Sequence (5′-3′) | Description |
|---|---|---|
| 1339-F1 | CATCATATGGAAAACTCCTCCTTAAGATTTATAT | Amplify Cac1339 promoter |
| 1339-R1 | ACCGCGGCCGCTTTATATTTAGTCCCTTGCCTTGCC | Amplify Cac1339 promoter |
| 1344-F1 | CATCATATGACATTAATAAATTAACTGTTATACT | Amplify Cac1344 promoter |
| 1344-R1 | ACCGCGGCCGCTTTTAAAACCCCTTCCCGAAATATT | Amplify Cac1344 promoter |
| 2612-F1 | CATCATATGAATCAAACCCCCTTAATTTTAAATA | Amplify Cac2612 promoter |
| 2612-R1 | TACCGCGGCCGCAATTATATATTTTATGTGTAGAAT | Amplify Cac2612 promoter |
| pCB102-F | TCCGGCGCGCCGATAATTTACAGAAAAGAAAATTA | Amplify pCB102 replicon |
| pCB102-R | TTCGGCCGGCCTGCAGCACATTAAGTATATACTATT | Amplify pCB102 replicon |
| catP-INV-F1 | TATTGTATAGCTTGGTATCATCTCATCATATATCCCCAATTCACC | For inverse PCR |
| catP-INV-R1 | TATTTGTGTGATATCCACTTTAACGGTCATGCTGTAGGTACAAGG | For inverse PCR |
| catP-Sou-F1 | GATTGTTTCCATACCGTTGC | For southern probe synthesis |
| catP-Sou-R1 | AGTTATTAAGTCGGGAGTGC | For southern probe synthesis |