Skip to main content
. 2016 Feb 2;7(13):15481–15491. doi: 10.18632/oncotarget.7139

Figure 2. Effect of PCGEM1 on castration resistance.

Figure 2

A. Relative expression of AR, AR variants (AR-v) and AR3 in LNCaP, LNCaP95 and CWR22Rv1 cells, as detected by Western (left) using N-terminal AR antibody or AR3 specific antibody; and AR3 mRNA levels, as detected by qRT-PCR (right) using AR3 specific primers (AR3-RT-5.1 and AR3-RT-3.1). β-actin was used as an internal control. B. Expression of PCGEM1 by qRT-PCR in LNCaP, LNCaP95 and CWR22Rv1 cells. C. PCGEM1-shRNA suppresses CWR22Rv1 cell growth. The cells were grown in androgen-free medium and the cell growth was measured for 4 days by MTT. D. PCGEM1-shRNA reduces tumor growth in the xenograft mouse model. CWR22Rv1 cells were injected into castrated male SCID mice subcutaneously as described in the text, and tumor growth was measured 7 days after injection. Tumors were harvested and weighted at day 31 after injection of tumor cells. 1, Ctrl shRNA; 2, PCGEM1-shRNA. Values in A, B and D are means ± SE (n = 3). **, p < 0.01, two-sided two-sample t test.