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. 2016 Feb 2;7(13):15481–15491. doi: 10.18632/oncotarget.7139

Figure 3. Androgen deprivation induces PCGEM1 expression and its subcellular re-localization.

Figure 3

A. AD induces PCGEM1 in LNCaP cells. The cells were cultured in the presence or absence of androgen for 7 days before harvesting for qRT-PCR. B. Reversible regulation of PCGEM1 and AR3 in LNCaP95 cells in response to AD. The cells that were originally maintained in androgen-free were switched to medium supplemented with 10 nM R1881 and grown for 2 days. The cells were then harvested for qRT-PCR (left) to detect PCGEM1 and AR3 RNA or Western (right) to detect AR3 at the protein level. C. Subcellular distribution of PCGEM1 in response to androgen. PCGEM1 was detected by FISH and AR was detected by IF. LNCaP cells were grown in androgen containing medium (top) or in androgen free medium for 2 days (bottom) before fixation for FISH and IF. D. Subcellular distribution of PCGEM1 in response to AD. Cells were fixed and PCGEM1 was detected by FISH. Only day 0 was shown for CWR22Rv1 because the distribution of PCGEM1 was same from day 0, day7 and day 28. Values in A and B are means ± SE (n = 3). **, p < 0.01, two-sided two-sample t test.