Figure 4. Regulation of AR3 by hnRNP A1 and PCGEM1.

A. Increasing AR3 expression by A1 winner, hnRNP A1-siRNA or hnRNP A1 knockout. CWR22Rv1 cells were transiently transfected with A1 winner oligo or hnRNP A1-siRNAs and were then harvested 2 days later for Western. hnRNP A1 knockout was made through CRISPR/Cas9 as described in the text. B. Suppression of PCGEM1 by RNAi reduces AR3 expression in CWR22Rv1 cells.