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. 2016 Feb 2;7(13):15481–15491. doi: 10.18632/oncotarget.7139

Figure 6. Co-localization of PCGEM1 and U2AF65 in nuclear speckles and the role of the binding sites of hnRNP A1 and U2AF65 in AR3 expression.

Figure 6

A. U2AF65 and PCGEM1 are co-localized in nuclear speckles in response to AD in LNCaP cells (left). However, AD has no effect on this co-localization in CWR22Rv1 cells (right). B. Reversible nuclear speckle localization of PCGEM1 in LNCaP95 cells in response to androgen. The cells were maintained in androgen-free medium and then switched to androgen-containing medium for 2 days. C & D. Binding sites for hnRNP A1 and U2AF65 in AR pre-mRNA are important to AR3 expression. CWR22Rv1 cells were transfected with mini-gene cassette reporters as indicated (C) and mCherry signals were examined 24 h after transfection. Relative expression of mCherry signals by randomly examining 10 fields and then normalized as 100% for AR3 wild type reporter. Values in (D) are means ± SE (n = 3). **, p < 0.01, two-sided two-sample t test.