Figure 10. Effect of clofibrate on cell cycle regulatory enzymes.
Cell lysates prepared from A. SUM149PT and B. SUM1315MO2 cells that were untreated or treated with 20 μM clofibrate for 24 h and 48 h and Western blotted for p53, p21, cyclin D1, cyclin E, and cyclin A. The blots were re-probed with anti-Tubulin antibody to confirm equal loading. A representative blot from three independent experiments is shown.