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. 2015 Nov 26;7(13):15577–15599. doi: 10.18632/oncotarget.6402

Figure 6. Effect of clofibrate treatment on the 5-lipooxygenase pathway in SUM149PT and SUM1315MO2 cells.

Figure 6

A. SUM149PT and SUM1315MO2 cells were untreated or treated with 20 μM clofibrate and cell lysates were prepared. These cell lysates were Western blotted for 5LO, LTA4H, and LTB4R, and then stripped and re-probed with anti-GAPDH antibody to confirm equal loading. B. Effect of clofibrate treatment on LTB4 secretion in SUM149PT and SUM1315MO2 cells. SUM149PT and SUM1315MO2 cells were untreated or clofibrate treated for 24 h and 48 h, and supernatants were collected for LTB4 quantification. Percent inhibition in LTB4 secretion was calculated by considering the secretion from untreated breast cancer cells as 100%. Each bar represents the average +/− SD from three independent experiments. ** represents statistically highly significant.