Table 1. Sequences of real time primers used in the study.
Primer | Orientation | Sequence (5′-3′) |
---|---|---|
GAPDH | Sense | GAAGGTGAAGGTCGGAGTC |
Antisense | GAAGATGGTGATGGGATTTC | |
18S | Sense | AACCCGTTGAACCCCATT |
Antisense | CCATCCAATCGGTAGTAGCG | |
HPRT | Sense | GGACAGGACTGAACGTCTTGC |
Antisense | CTTGAGCACACAGAGGGCTACA | |
HMG-CoA Synthase | Sense | GAATCAGTGGAAGCAAGCTGG |
Antisense | GAATCAGTGGAAGCAAGCTGG | |
CPT1a | Sense | TCCAGTTGGCTTATCGTGGTG |
Antisense | CTAACGAGGGGTCGATCTTGG | |
SREBP-1c | Sense | GGAGCCATGGATTGCACATT |
Antisense | GCTTCCAGAGAGGAGGCCAG | |
SREBP-2 | Sense | CCCTTGACTTCCTTGCTGCA |
Antisense | GCGTGAGTGTGGGCGAATC | |
Acyl CoA Oxidase | Sense | AGTGCCCAGATGATCTTGAAGC |
Antisense | CTGCCAGAGGTAACCATTTCCT | |
SCD-1 | Sense | TGGGTTGGCTGCTTGTG |
Antisense | GCGTGGGCAGGATGAAG | |
FASN | Sense | CTTCCGAGATTCCATCCTACGC |
Antisense | TGGCAGTCAGGCTCACAAACG | |
PPAR-α | Sense | CTATCATTTGCTGTGGAGATCG |
Antisense | AAGATATCGTCCGGGTGGTT |