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. 2016 Jan 13;7(13):15618–15631. doi: 10.18632/oncotarget.6905

Figure 7. LILRB3-Fc binding of MCF-7 cells following shRNA silencing of the expression of cytokeratin 8 as assessed by confocal microscopy.

Figure 7

A. LILRB3*12-Fc binding colocalised with the binding of the mAbs 1E8 (anti-cytokeratin 8) and DA-7 (anti-cytokeratin 18) in permeablised MCF-7 cells that were previously transduced with a non gene-specific shRNA construct. shRNA constructs were used to stably silence the expression of cytokeratin 8 in MCF-7 epithelial cells. Binding of both mAbs and LILRB3*12-Fc were greatly reduced in MCF-7 cytokeratin 8 knock down cells (CK8 KD). Bar represents 10μm. B. MCF-7 cells that were previously transduced with a non gene-specific shRNA construct were stained with either: i) the 1E8 mAb and Fc negative control construct, or ii) isotype control mAb and the LILRB3*12 Fc fusion. Both the isotype and control Fc displayed minimal binding. LILRB3*12-Fc staining did not co-localise with phalloidin staining (actin, panel iii) or DM1A mAb (anti-tubulin, panel iv).