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. 2016 Feb 18;7(13):15811–15827. doi: 10.18632/oncotarget.7515

Figure 4. ARF1 is important for the maintenance of adherent junctions.

Figure 4

A. MCF7 cells were transfected with empty vector (cnt), ARF1 or ARF6 and fixed after 48 hours. Cells were stained for β-catenin, E-cadherin and nuclei with Hoechst. Images are from three independent experiments, with more than 100 cells per condition. Scale bars, 10 μm. B. Cells transfected as in A. were used to prepare membrane fractions. Associated β-catenin, E-cadherin, ARF1 and ARF6 were assessed by Western blotting. These experiments are representative of three others. β1-Integrin was used as a plasma membrane marker, GAPDH as a cytosol marker and Histone 3 as nuclei marker. C. Cells transfected as in A. were stimulated with FBS (10%) for four hours. Endogenously expressed β-catenin, HA-tagged (hemagglutinin) proteins and actin were detected by Western blotting. Data are the mean ± SEM of four experiments. Statistical analysis was performed using a two-ways ANOVA followed by a Bonferroni's multiple comparison test. **p < 0.01, ****p < 0.0001.