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. 2016 Feb 17;7(13):16090–16103. doi: 10.18632/oncotarget.7449

Figure 2. SULF2 mediates IR-induced cell invasion.

Figure 2

A. A549 cells treated with control RNA or 2 sets of SULF2 siRNA were irradiated with 10 Gy of γ-rays. After a 24-h incubation, cell invasiveness was compared on Matrigel-coated polycarbonate filters (top). The levels of STAT3, phosphorylated STAT3, Bcl-XL, and SULF2 were compared by western blotting (WB) or RT-PCR (bottom). B. Left, A549 cells were transfected with the empty pcDNA3 vector or the vector encoding SULF2. After a 48-h incubation, the levels of SULF2, STAT3, phosphorylated STAT3, and Bcl-XL were compared by RT-PCR or western blotting. Right, Cells were transfected with expression vectors and siRNAs in the indicated combinations, and cellular invasiveness and protein levels of STAT3 and Bcl-XL were compared.