Figure 5. Sublethal doses of IR increase SULF2 mRNA levels in vivo.
A. H460 cells were implanted to form xenograft tumors in mice. On the 7th day after implantation, the tumors were locally irradiated with the indicated doses of γ-rays. Tumor diameters were measured, and tumor volumes were calculated on the specified days. B. At 1, 2, or 5 days post-irradiation, the mice were sacrificed and the tumors were removed. SULF2 mRNA levels in the tumors were compared by RT-PCR. The values shown represent the relative levels of SULF2 mRNA normalized to those of GAPDH.