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. 2016 Feb 21;7(13):16146–16157. doi: 10.18632/oncotarget.7550

Figure 4. Soluble Tie2 expression counters the pro-invasive phenotype upon anti-VEGF therapy.

Figure 4

(A) GL261- and GL261. sTie2-derived tumor sections from mice treated with DC101 or vehicle were stained with hematoxylin and eosin. Invasive features were observed in Gl261-bearing mice treated with DC101 but not in GL261.sTie2bearing mice treated with DC101. Scale bars = 20 μm. N, normal brain; T, tumor. (B) Quantification of invasive nodules present in intracranial syngeneic tumors after DC101 treatment. GL261 and GL261.sTie2 were tested. Data are presented as mean ± SD. ***P < 0.001. (C) Representative images of Tie2 (red) and Iba1 (green) double immunofluorescence in sections from GL261 and GL261.sTie2 syngeneic tumors treated with DC101 or vehicle. DAPI was used for nuclear staining (blue). White arrows indicate the presence of Iba1+Tie2+ cells. Scale bars = 20 μm. (D) Quantification of TEMs (Tie2+Iba1+ cells) present in a high-power field (HPF) after DC101 treatment. Data are presented as mean ± SD. ns, P > 0.05; *P < 0.05; ***P < 0.001.