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. 2016 Feb 18;7(13):16172–16179. doi: 10.18632/oncotarget.7473

Figure 4. Plk5 mRNA perturbation during cell cycle and its protein expression support that Plk5 is a tumor suppressor.

Figure 4

A. With microfluidic devices, transcriptomes of individual Hela cells were arranged by similarity to reveal the perturbations of individual genes at different stages of a cell cycle. The mRNA perturbation of Plks (Plk1-5) during a cell cycle is plotted along a cell cycle. Unlike Plk1-4, Plk5 mRNA is highly expressed right before entering S phase and rapidly down-regulated (blue arrow). This perturbation pattern is typical for S-phase check point genes. B. Agreeing with previous studies, immunostaining shows that Plk5 is induced by DNA damage in the primary tumor (PT) when compared with normal tissue (NT). However, Plk5 protein is less at lymphatic metastasis (LM) suggest more cells lost the Plk5 check-point before entering S phase. Relative intensity is quantified with Imagej.