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. 2016 Feb 22;7(13):16180–16193. doi: 10.18632/oncotarget.7568

Figure 2. Overexpression of LATS1 inhibits cell growth and invasion.

Figure 2

(A) LATS1 mRNA and (B) protein expression were respectively detected by Real-time PCR and Western blotting in SGC-7901 and HGC-27 cells transfected with LATS1. (C) The effect of LATS1 overexpression on cell proliferative activity was estimated by MTT assay, indicating that overexpression of LATS1 reduced cell growth after LATS1 transfection for 72 h. (D) Representative micrographs of Transwell invasion assay were indicated in SGC-7901 and HGC-27 cells transfected with LATS1 (magnification, 200×). (E) Overexpression of LATS1 decreased cell invasive potential compared with the NC group. (F) Flow cytometry analysis of early apoptotic rate in SGC-7901 and HGC-27 cells after LAST1 transfection for 48 h. (G) Overexpression of LATS1 induced cell apoptosis compared with the NC group. (H) Flow cytometry analysis of cell cycle distribution in SGC-7901 and HGC-27 cells transfected with LATS1. (I) Overexpression of LATS1 induced cell cycle arrest at S phase compared with the NC group (**P < 0.01). The data were shown as the means ± SD of three independent experiments. **P < 0.01.